Restriction enzymes are hugely particular nucleases which take place ubiquitously between prokaryotic organisms, the place they serve to guard bacterial cells opposed to overseas DNA. many differing kinds of restrict enzymes are identified, between them multi-subunit enzymes which rely on ATP or GTP hydrolysis for goal website position. the easiest recognized representatives, the orthodox variety II restrict endonucleases, are homodimers which realize palindromic sequences, four to eight base pairs in size, and cleave the DNA inside or instantly adjoining to the popularity web site. as well as their very important organic function (up to ten % of the genomes of prokaryotic organisms code for restriction/modification systems!), they're one of the most vital enzymes used for the research and recombination of DNA. furthermore, they're version structures for the learn of protein-nucleic acids interactions and, as a result of their ubiquitous occurence, additionally for the knowledge of the mechanisms of evolution.
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