PCR Cloning Protocols, moment variation, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the latest systems for DNA cloning and mutagenesis. the following the researcher will locate simply reproducible tools for the entire significant points of PCR use, together with PCR optimization, computing device courses for PCR primer layout and research, and novel diversifications for cloning genes of specific features or starting place, with emphasis on lengthy distance PCR and GC-rich template amplification. additionally incorporated are either traditional and novel enzyme-free and restrict site-free techniques to clone PCR items right into a variety of vectors, in addition to cutting-edge protocols to facilitate DNA mutagenesis and recombination, and to clone the tough uncharacterized DNA flanking a identified DNA fragment.
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