Download E-books Der Experimentator Molekularbiologie / Genomics (German Edition) PDF

By Cornel Mülhardt

Dieses Buch enthält das Grundlagenwissen sowie Tipps und tips für den Umgang mit Nucleinsäuren. Der Autor kennt Lust und Frust der täglichen Laborroutine ganz genau.

  • Präparieren, Fällen, Konzentrieren und Reinigen von Nucleinsäuren
  • Restriktionsenzyme, Gele, Blotten
  • Polymerase-Kettenreaktion
  • RNA-Isolierung, -Transkription
  • Klonierung von DNA-Fragmenten
  • Markierung von Sonden, Hybridisierung, Screening, Sequenzierung
  • Mutagenese, In-vitro-Translation, transgene Mäuse, Transgenexpression, Gentherapie, Genomik

Dieses Buch richtet sich an alle Experimentatoren, die molekularbiologische Versuche durchführen wollen und gern nachvollziehen möchten, was once sich in ihrem Reaktionsgefäß abspielt. Das ganze Spektrum der üblichen molekularbiologischen Methoden wird vorgestellt, kommentiert und Alternativen aufgezeigt.

Der lockere Ton wendet sich gleichermaßen an Studenten wie an BTAs und Laboranten, aber auch der alte Hase wird hier und dort noch etwas Neues entdecken. Die 7. Auflage wurde überarbeitet und aktualisiert.

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