By Roger L. Lundblad
Revised and up to date, Chemical Reagents for Protein amendment, 3rd variation is an encyclopedic paintings describing the numerous methods to the site-specific amendment of proteins. greater than 2,000 references are stated describing the improvement of a number of the reagents. This publication explores how using site-specific protein amendment pertains to a large choice of biotechnology strategies and items, equivalent to protein microarrays, hydrogels, controlled-release prescription drugs, and biotherapeutics, and proteomics.
With chapters targeting analyses of person amino acids, extra chapters disguise chemical cross-linking and the chemical cleavage of peptide chains. there's substantial connection with using site-specific chemical amendment in proteomics. the writer will pay specific cognizance to the final environmental components that impact the reactivity of person amino acid residues. He offers innovations for the characterization of changed proteins, together with mass spectrometry, and contains elevated insurance of spectroscopic thoughts and crystallographic analyses. this can be through insurance of nitrosylation reactions and different non-enzymatic amendment techniques. particular reference is given to the differential labeling of protein sensible teams with reagents classified with sturdy isotopes. assets comprise an inventory of present journals that submit site-specific chemical amendment reports, the main universal reagents and their houses, and a touch checklist of reagent suppliers.
Chemical Reagents for Protein amendment, third version offers the main often used tools for the site-specific chemical amendment of proteins, suggestions for protein characterization, designated laboratory info for components that impression reactivity and reproducibility, and industry-specific assets.
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